CELLS OF THE IMMUNE SYSTEM.
OMICS TECHNOLOGIES

Flow Cytometry Gating

Flow cytometry gating is the selection and analysis of cell subpopulations of interest in multicolor flow cytometry. Typically, the first step of gating includes sorting cells by fluorochrome-targeted CD45 and lymphocytes in forwarding and orthogonal scattered light. Next, a proportion of distinct T-cell subsets is measured since each of them is targeted by a specific fluorochrome.
In the course of immune responses, T cells acquire a new phenotype and cytokine profile that can be defined using the following markers:
CD45 is Leukocyte Common Antigen;
CD27 represents a cell adhesion molecule related to the TNF receptor superfamily, which is responsible for the survival of activated T cells or their apoptosis;
CD28 is a
costimulatory molecule is adaptive T-cell immune responses;
CD62L is a cell adhesion molecule related to the selectin family that plays a role in the first stage, "rolling" action of leukocute transmigration through endothelial cells;
CCR7 is a chemokine receptor for chemokines CCL19 (ELC) and CCL21 (SLC) that promote T-cell migration to secondary lymphoid organs; and
Cytokines secreted by T cells.секретируемые клетками.

Marker

Naïve T cells

Effector T cells

Memory T cells

TCM

TEM

 CD45 (isoforms)

 CD45RA+,  CD45RO-

 CD45RA+,  CD45RO-

 CD45RA-,  CD45RO+

 CD45RA-,  CD45RO+

 CD27, CD28  

 CD27+, CD28+

 CD27-, CD28-

 CD27+, CD28+

 CD27+, CD28+  (there are different  expression types)

 CD62L

 CD62L+

 CD62L-

 CD62L+

 CD62L-

 CCR7

 CCR7+

 CCR7+

 CCR7+

 CCR7-

 Cytokines

 IL-2

 IFN-γ, TNF-β

 IL-2

 IFN-γ, TNF-β

Researchers sometimes get different data concerning the phenotype of T-cell subsets. In addition, new information is gradually accumulated on the heterogeneity of T-cell subpopulations.

©V.V.Klimov